Part:BBa_K4645003:Design
Fusion protein formed by CsgA and Hsa with (GGGGS)4 as linker.
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 37
Illegal PstI site found at 861 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 37
Illegal PstI site found at 861 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 37
Illegal PstI site found at 861 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 37
Illegal PstI site found at 861 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Considering the codon bias in Escherichia coli, we optimized our fusion protein sequence using the online codon optimization tool from GenScript.
Source
CsgA:https://www.ncbi.nlm.nih.gov/gene/949055 Hsa:https://www.ncbi.nlm.nih.gov/nuccore/AB029393
References
[1]Bensing BA, Stubbs HE, Agarwal R, Yamakawa I, Luong K, Solakyildirim K, Yu H, Hadadianpour A, Castro MA, Fialkowski KP, Morrison KM, Wawrzak Z, Chen X, Lebrilla CB, Baudry J, Smith JC, Sullam PM, Iverson TM. Origins of glycan selectivity in streptococcal Siglec-like adhesins suggest mechanisms of receptor adaptation. Nat Commun. 2022 May 18;13(1):2753.
[2]Coffey BM, Anderson GG. Biofilm formation in the 96-well microtiter plate. Methods Mol Biol. 2014;1149:631-641.
[3]David J. Lynch, Tracey L. Fountain, Joseph E. Mazurkiewicz, Jeffrey A. Banas, Glucan-binding proteins are essential for shaping Streptococcus mutans biofilm architecture, FEMS Microbiology Letters, Volume 268, Issue 2, March 2007, Pages 158–165.